Product Description
Use:Anti D (IgG) Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for Rh typing and is for in-vitro use only. For direct and indirect antiglobulin test.
Principle:Human red cells are classified as Rh positive (Rh +) or Rh negative (Rh -) depending upon the presence or absence of 'D antigen on them. Major percentage of the population is Rh positive. Human red blood cells possessing D antigen will agglutinate in the presence of corresponding antibody. The red blood cells are first checked using Anti-D (IgM) monoclonal antibodies. Agglutination of red cells with Anti- D (IgM) monoclonal antibodies indicates the presence of D-antigen and hence Rh positive result. If no agglutination is obtained then the red blood cells are checked with Anti-D (IgG) monoclonal antibodies as it sensitize the Rh positive/ Du positive cells which will give agglutination on addition of coombs reagent (Indirectcoombs technique).Anti A-Monoclonal Antibodies:Intended UseAnti-A Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for ABO grouping. The reagent is for in-vitro use only
PrincipleThe ABO Grouping is defined both by the presence of 'A and/or B antigens on the surface of the red blood cells and by the simultaneous presence of anti-A and/or anti-B antibodies in the serum. An individual has in his serum the antibodies corresponding to the antigens which are not on his red blood cells. It is thus necessary to identify the erythrocyte antigens by the known ANTI- A, ANTI- B and ANTI- AB monoclonal antibodies(forward typing) and then confirm the results by verifying the presence of corresponding antibodies in the blood serum to be tested with control cells:A1,'A2, 'B and 'O (Reverse typing). Human red blood cells possessing 'A and /or 'B antigen will agglutinate in the presence of antibody directed towards the antigen. For example agglutination of RBCs with Anti A monoclonal antibodies indicates the presence of 'A Antigen on the red blood cells.
Technical Details 
Quick and Accurate Blood GroupingJ Mitra Anti ABD Test Kit employs the agglutination reaction for reliable ABO and D blood typing, facilitating safe transfusion decisions. The ready-to-use reagents and simple procedure make it convenient for healthcare professionals to obtain results within minutes, ensuring speed and accuracy in critical environments such as hospitals and blood banks.
Comprehensive Kit with User-Friendly DesignThis slide test kit includes Anti-A, Anti-B, and Anti-D reagents, test cards, droppers, and clear instructions. Designed for everyday use, it is lightweight, easy to handle, and disposable, which helps maintain hygiene and reduce the risk of contamination during blood group testing procedures. Its portability further adds to its practicality.
FAQ's of J Mitra Anti Abd test kit:
Q: How does the J Mitra Anti ABD Test Kit detect blood group antibodies?
A: This kit uses the slide agglutination method, where Anti-A, Anti-B, and Anti-D reagents are mixed with a blood sample. If agglutination occurs, the blood group is identified, providing qualitative results for ABO and D typing.
Q: What is the process for using the J Mitra Anti ABD Test Kit?
A: To use, place a drop of each reagent and the blood sample onto the test card, mix gently using the provided droppers, and observe for agglutination. Results are visible within two minutes, making the process fast and efficient.
Q: When should I use this test kit, and what kind of sample is required?
A: The kit is ideal for blood grouping before transfusions or organizing blood donations. It requires either whole blood or red blood cells as the test sample.
Q: Where can the J Mitra Anti ABD Test Kit be used?
A: This kit is suitable for use in hospitals, blood banks, and laboratories. Its portable and manual design enables deployment in various medical and laboratory settings across India and other regions.
Q: What are the benefits of using the J Mitra Anti ABD Test Kit?
A: Benefits include rapid and reliable blood group detection, user-friendly operation, ready-to-use reagents, and single-use components for safety. Its portability and quick result time make it ideal for urgent clinical situations.
Q: How should I store the kit and what is its shelf life?
A: Store the kit at 2C to 8C, ensuring it is not frozen. The shelf life is 12 to 18 months from the date of manufacture, maximizing usability and reducing waste.