Product Description
The TRUSTwell HBsAg ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of hepatitis B virus surface antigen (HBsAg) in human serum or plasma.
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Based on double Ab-Sandwich immunoassay technique ensure high specificity
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Solid microwells pre-coated with monoclonal anti-HBsAg antibodies
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Liquid conjugates composed of polyclonal anti-HBsAg Ab conjugated with HRP
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Relative sensitivity: 100% and specificity: 99.78 %
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Comparable accuracy to the leading other ELISA kits
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Clear borderlines with high accuracy positive and negative controls
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Breakaway microwell strips
Reliable Detection of HBsAgTRUSTwell HBsAg ELISA Kit uses the Sandwich ELISA technique to detect Hepatitis B surface antigen in human plasma or serum samples. This method ensures robust sensitivity and specificity, making it an essential tool for routine screening and diagnosis. The kit is CE marked and meets WHO standards, providing confidence and regulatory assurance for clinical laboratories.
Convenient and User-Friendly FormatDesigned with ease of use in mind, all components are ready to use and clearly labeled. The test can be completed in approximately 60 minutes, thanks to streamlined protocols. Both positive and negative controls are included, supporting result accuracy. The kit is packed in sterile, medical-grade plastic and features user-friendly instructions to facilitate smooth, error-free operation.
Storage and Shelf LifeFor optimal performance, store all kit components at 2C - 8C; avoid freezing to maintain reagent integrity. The kit remains effective for 12 months from the manufacturing date when stored as recommended. This long shelf life supports flexibility for laboratories and clinics handling variable sample volumes.
FAQ's of TRUSTwell HBsAg ELISA Kit:
Q: How does the TRUSTwell HBsAg ELISA Kit detect Hepatitis B surface antigen?
A: The TRUSTwell HBsAg ELISA Kit employs a sandwich ELISA principle, in which antibodies on a microplate capture HBsAg present in plasma or serum samples. After binding specific reagents and substrates, a colorimetric change is measured at 450nm, allowing qualitative determination of HBsAg presence.
Q: What sample types are compatible with this kit?
A: The kit is validated for use with human serum and plasma samples, ensuring versatility in clinical practice and routine laboratory workflows.
Q: When and how should the kit be stored?
A: All kit components should be stored refrigerated at 2C - 8C and must not be frozen, as freezing can compromise reagent performance. Proper storage guarantees effectiveness until the stated 12-month shelf life from manufacturing.
Q: Where can the kit be used?
A: This ELISA kit is suitable for hospitals, diagnostic laboratories, research settings, and other healthcare facilities where HBV screening is required. Its portable and disposable format supports diverse environments.
Q: What is included in the TRUSTwell HBsAg ELISA Kit package?
A: Each kit provides a microplate, sample diluent, wash buffer, conjugate, TMB substrate, stop solution, positive and negative controls, and detailed instructions for use. All components come in medical-grade, sterile packaging.
Q: What are the benefits of using this kit compared to alternative methods?
A: The TRUSTwell HBsAg ELISA Kit offers reliable, rapid results with high sensitivity and specificity that fulfill WHO and CE standards. It is easy to use-requiring no real-time instrumentation-and provides high throughput for batch testing.
Q: How long does the assay take to complete and what equipment is required?
A: The total assay time is approximately 60 minutes. Results are read using a microplate reader set at 450nm, which allows for clear qualitative interpretation of the HBsAg detection.