Product Description
4TH Generation Microlisa HIV Ag & Ab is an in- vitro qualitative enzyme immunoassay for the detection of antibodies to HIV-1 and/or HIV-2 and HIV-1 p24 antigen in human serum or plasma. It is intended for screening of blood donors or other individuals at risk for HIV-1 and/or HIV-2 infection and for clinical diagnostic testing.
Salient Features - Use of Envelope Antigens gp41, C terminus of gp120 for HIV-1 and gp36 for HIV-2 & simultaneously detection of p24 antigen with p24 monoclonal antibodies
- Detection of Group O & subtype C for HIV-1 prevalent in India
- Colour coded reagents to monitor the procedural steps
- Assay procedure: 120 mins
- Shelf life: 18 months at 2-8C
- Sensitivity 100% & Specificity 99.97%
- Significant reduction in window period
- Easy to use on automated and semi-automatic processor
Advanced HIV Screening TechnologyThe Microlisa HIV Ag and Ab Test Kit implements both sandwich and indirect ELISA methodologies, significantly enhancing its ability to detect both HIV-1 p24 antigen and antibodies to HIV-1 & HIV-2. Its third/fourth generation status ensures superior sensitivity and specificity, making it a preferred choice for laboratories aiming for reliable HIV diagnostics that meet international standards.
Comprehensive and Convenient KitEach kit is supplied as a ready-to-use package, including microplates, all necessary reagents, controls, and a detailed instruction manual. Designed for manual, laboratory-based use, it accommodates 96 samples per run and operates with a standard ELISA reader, making it suitable for high-throughput environments needing efficient and dependable HIV screening solutions.
FAQ's of J Mitra Microlisa HIV Ag and Ab test kit:
Q: How does the J Mitra Microlisa HIV Ag and Ab Test detect HIV in blood samples?
A: The test kit detects HIV by using a combination of sandwich and indirect ELISA principles to identify both HIV-1 p24 antigen and antibodies against HIV-1 and HIV-2 in human serum or plasma. If these are present in the sample, the kit's colored reaction signifies a positive result.
Q: What samples can be tested with the Microlisa HIV kit, and what equipment is required?
A: This kit is validated for use with human serum or plasma samples. A standard ELISA reader capable of reading absorbance at 450 nm is required to interpret the results after completion of the protocol.
Q: When should the Microlisa HIV kit be used during the screening process?
A: The kit is intended for use in the qualitative screening of blood, plasma, or serum samples for HIV-1 p24 antigen and antibodies to HIV-1 & HIV-2, often as the initial test in diagnostic algorithms per laboratory and regulatory guidelines.
Q: Where should the kit be stored and for how long is it usable?
A: Store the Microlisa HIV kit at 2-8C. The kit remains usable for 12 months from its manufacturing date; always check the batch-specific lot number and expiry on the box before use.
Q: What is the typical process and time required to run a test with this kit?
A: The testing process involves adding samples, controls, and reagents to the provided microplate, followed by incubations and washes as per the manual. The complete test requires approximately 2 hours before results can be read using an ELISA reader.
Q: What are the main benefits of using this kit for HIV screening?
A: The Microlisa kit offers high sensitivity and specificity (>99.5%), easy-to-use components, and supports early detection of both HIV antigen and antibodies. Its compliance with WHO standards assures reliable performance in a variety of laboratory settings.
Q: Is the kit single-use, and are the materials safe for disposal?
A: Yes, all microplates and reagents in the kit are intended for single use and are made from plastic and medical-grade materials. Follow your laboratory's biohazard disposal protocols after testing.