Product Description
The TRUSTwell HCV IgG ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of IgG to Hepatitis C virus (HCV) in human serum or plasma.
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Based on indirect immunoassay technique ensure high specificity
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Solid microwells pre-coated with recombinant HCV antigens
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Liquid conjugates composed of anti-human IgG conjugated with HRP
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Relative Sensitivity: 100 %, Specificity: 99.81 %
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Comparable accuracy to the leading other ELISA kits
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Clear borderlines with high accuracy positive and negative controls
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Breakaway microwell strips
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Evaluated by NIB
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Assay time: 90 Minutes
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12-months shelf life at 2-8 C
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Microwells coated with recombinant HCV antigens
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Sample diluent
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HCV negative control
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HCV positive control
High Sensitivity and AccuracyEngineered with a sensitivity greater than 99% and specificity exceeding 98%, this ELISA kit guarantees precise detection of HCV IgG antibodies. It minimizes false positives and negatives, making it ideal for effective screening and diagnosis in clinical laboratories.
User-Friendly Procedure for Quick ResultsThe kit's manual, colorimetric detection system is straightforward, with a primary incubation of 60 minutes followed by a 30-minute substrate reaction. All essential controls and an instruction manual are included, ensuring accurate interpretation for users of various proficiency levels.
Reliable and Comprehensive ContentsEach kit provides components for 96 tests, positive and negative controls, and ready-to-use reagents. The materials are disposable after single use, maintaining hygiene and simplifying post-procedure cleanup in the lab environment.
FAQ's of TRUSTwell HCV IgG ELISA Kit:
Q: How does the TRUSTwell HCV IgG ELISA Kit detect Hepatitis C Virus antibodies?
A: The kit uses the indirect ELISA technique to identify HCV IgG antibodies in human serum or plasma. When a sample contains these antibodies, they bind to antigens coated on the wells, resulting in a color change detected at 450 nm after substrate incubation.
Q: What types of samples can be used with this ELISA kit?
A: You can utilize either human serum or plasma samples for testing with the TRUSTwell HCV IgG ELISA Kit, ensuring versatility in clinical and diagnostic laboratory settings.
Q: When should the kit be used, and what is the typical incubation time?
A: It is designed for laboratory diagnostic use to screen for HCV IgG antibodies. The incubation time for the primary reaction is 60 minutes, followed by an additional 30 minutes for substrate development.
Q: Where should the TRUSTwell HCV IgG ELISA Kit be stored for optimal shelf life?
A: The kit must be kept at a storage temperature between 2C and 8C. Under these conditions, it maintains a shelf life of 12 months from the date of manufacture.
Q: What are the key benefits of using this ELISA kit for HCV detection?
A: The primary benefits include its high sensitivity and specificity, inclusion of necessary controls, ease of use with manual operation, and comprehensive instructions, all of which contribute to reliable, accurate diagnostic outcomes.
Q: How should used kit components be disposed of after testing?
A: All kit components are intended for single use and should be disposed of according to local laboratory biohazard waste procedures after testing to ensure safety and compliance.
Q: Is the TRUSTwell HCV IgG ELISA Kit suitable for real-time or portable detection?
A: No, the kit is not designed for real-time or portable use. It operates manually in a laboratory environment, with results analyzed by colorimetric reading and manual or cable data entry.