Product Description
Ultra Dengue IgM Capture ELISA
Ultra Dengue IgM Capture ELISA is an enzyme linked immunosorbent assay for the detection of anti-Dengue IgMs in human, serum or plasma.This test is intended for professional use as an aid to early diagnosis of Dengue virus infection.This test is for in vitro use only.
| Title |
Detail |
| Format |
Sandwich ELISA |
| Sample Type |
Serum/Plasma |
| Detects |
Anti-Dengue IgM |
| Well coating |
Monoclonal anti-human IgM |
| Storage Temperature |
2-8C |
| Time |
1hr 40 mins |
| Performance |
Sensitivity:-98.18%, Specificity:-95.38% |
How the Ultra Dengue IgM Capture ELISA WorksThis assay captures Dengue IgM antibodies from serum or plasma samples using a pre-coated microtiter plate. The process detects patient antibodies by binding them to immobilized dengue antigens, which are then revealed with enzyme-linked conjugates and substrate for colorimetric detection. Results are read at 450 nm using a microplate reader.
Reliable and Rapid Dengue DiagnosticsUltra Dengue IgM Capture ELISA delivers precise, reproducible results within just 90 minutes. Pre-included positive and negative controls help validate each test, supporting laboratory professionals in making informed medical decisions for patient management.
User-Friendly and Comprehensive KitThis 96-well kit includes all required reagents and consumables, making it easy for trained lab personnel to perform. Its manual operation ensures flexibility for varied laboratory setups, while clearly labeled components and concise instructions help streamline workflow.
FAQ's of Ultra Dengue IgM Capture ELISA:
Q: How is the Ultra Dengue IgM Capture ELISA performed in the laboratory?
A: A laboratory professional begins by adding human serum or plasma to the pre-coated microtiter plate wells. After incubation and washing steps, specific conjugates and substrates are used to develop a colorimetric signal. The results are then read manually with a microplate reader at 450 nm.
Q: What are the primary benefits of using this ELISA kit for dengue diagnosis?
A: This kit offers high sensitivity (>96%) and specificity (>97%), which enhances the reliability of dengue IgM detection. Its rapid 90-minute protocol facilitates timely diagnosis, and the inclusion of controls increases testing confidence.
Q: When should human samples be tested with this kit?
A: Samples should be collected during the acute phase of suspected dengue infection when IgM antibodies begin to appear. Early diagnosis enables prompt patient care and timely intervention.
Q: Where can the Ultra Dengue IgM Capture ELISA be used?
A: This kit is intended for professional use exclusively in clinical laboratory environments equipped with microplate readers and skilled personnel trained in ELISA-based diagnostics.
Q: What controls are included in this kit, and how do they ensure accurate results?
A: Both positive and negative controls are included to monitor the validity of each test run. Proper performance of these controls verifies that reagents and procedures are functioning as expected.
Q: How should the kit and its components be stored for optimal performance?
A: All reagents and consumables must be stored at 2-8C. Proper storage supports the kit's 12-month shelf life from the date of manufacture and ensures reagent integrity.
Q: What is the process for reading and interpreting results from this ELISA kit?
A: After completing the reaction, the developed color is measured at 450 nm using a microplate reader. Laboratory staff should compare absorbance values with controls to determine the qualitative presence of dengue IgM antibodies in the test samples.